Abstract

Organic dirt on touch surfaces can be biological contaminants (microbes) or nutrients for those but is often invisible by the human eye causing challenges for evaluating the need for cleaning. Using hyperspectral scanning algorithm, touch surface cleanliness monitoring by optical imaging was studied in a real‐life hospital environment. As the highlight, a human eye invisible stain from a dirty chair armrest was revealed manually with algorithms including threshold levels for intensity and clustering analysis with two excitation lights (green and red) and one bandpass filter (wavelength λ = 500 nm). The same result was confirmed by automatic k‐means clustering analysis from the entire dirty data of visible light (red, green and blue) and filters 420 to 720 nm with 20 nm increments. Overall, the collected touch surface samples (N = 156) indicated the need for cleaning in some locations by the high culturable bacteria and adenosine triphosphate counts despite the lack of visible dirt. Examples of such locations were toilet door lock knobs and busy registration desk armchairs. Thus, the studied optical imaging system utilizing the safe visible light area shows a promising method for touch surface cleanliness evaluation in real‐life environments.

Highlights

  • Touch surfaces are an important source of bacteria and many pathogenic and opportunistic bacteria can persist on a surface even for months, these contaminated surfaces can further contribute to transmission of pathogens that can even cause hospital acquired infections [1, 2]

  • Given that infectious doses can be extremely low, for example, less than 10 spores or colony-forming units (CFU), the detection limit of bacteria is crucial in the infection control in hospital environment [6]

  • The results suggest that the sampling location affected significantly (P < .05) for culturable bacteria count and adenosine triphosphate (ATP) levels (Table 1) in studied surfaces

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Summary

| INTRODUCTION

Touch surfaces are an important source of bacteria and many pathogenic and opportunistic bacteria can persist on a surface even for months, these contaminated surfaces can further contribute to transmission of pathogens that can even cause hospital acquired infections [1, 2]. The important biological contaminants vary depending on the environment; in the hospital environment, many antibiotic-resistant strains nowadays pose challenges such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and Clostridium difficile [1, 3] Despite these challenges, a real-time monitoring system is still lacking to indicate biological contamination on touch surfaces and current methods include fast but unspecific. The aim of this study was to study hyperspectral imaging further in real-life environment and to confirm the usability of this system (AutoDet) for detecting visible and invisible stains to the human eye on touch surfaces in a hospital. Both manual algorithms/methods as well as machine vision algorithms were used

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Findings
| DISCUSSION
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