Contamination and subsequent multiplication of soft rot erwinias on healthy potato leaves and debris after haulm destruction

Abstract

Small plots of potatoes were inoculated with a mixture of Erwinia carotovora (E. c.) subsp. carotovora and E. carotovora subsp, atroseptica strains resistant to rifampicin. Subsequently the population off, c. subsp, carotovora and E. c. subsp, atroseptica (rifampicin‐resistant and wild types) present as epiphytes on the surface of potato leaves was assessed using three methods, qualitative, semi‐quantitative and quantitative, during 1986 and 1987. The population was generally low (< 102 colony forming units (> 104cfu/g leaves) but reached higher levels (> 104 cfu/g) on occasions later in the growing season, Rifampicin‐resistant erwinias were reisolated only infrequently throughout this study. Different methods of haulm destruction (herbicide, pulverization, sulphuric acid treatment and natural senescence) greatly influenced the number of erwinias present in the resulting plant debris. Pulverization resulted in the highest population (106‐107 wild‐type cfu/g) in both seasons. In 1987. the wettest of the two seasons of this study, herbicide treatment resulted in similarly high populations. The results suggest that the high numbers of erwinias found in the haulm debris were probably derived from the generally low populations of epiphytic bacteria previously present on healthy leaves, E. c. subsp, carotovora was the most frequent subspecies in the rotting plant debris; E. c. subsp, atroseptica was more commonly found on healthy leaves. The implications of the results are discussed in relation to the production of seed potatoes with a low risk of blackleg.