Contagious Ektima (CE) aşısının bağışıklık ve zararsızlık çalışmalarında tavşanların kullanılması

Abstract

Contagious ecthyma (CE) is a zoonotic viral infection and common localized cutaneous infection of young sheep and goats caused by a Parapoxvirus with worldwide distribution. The aim of this study was to use rabbits instead of lambs and kids in immunity and safety studies after production of the CE vaccine. The titres of Pendik CE pathogen strain isolated lamb (E(P)CK4) and attenuated vaccine strain originated lamb (E(P)CK22) used in the study were TCID50 106.50 and 107.00/ml, respectively. In the study to determine the pathogenicity of CE virus (E(P)CK4) in rabbits, it was found that CE virus-specific hyperemia, vesicles and pustules were not observed and did not contain CE virus by PCR test in the scabs in the scarred back-waist regions, and body temperature did not increase in rabbits. In the blood sera of rabbits treated with pathogen CE virus and vaccinated with CE vaccine virus, no antibodies were detected against CE virus. It was found that the vesicles, pustules and scabs appeared on day 3 and the lesions healed on the 15th day. In the immunity study in lambs and kids vaccinated with CE vaccine (E(P)CK22), it was found that CE vaccine virus protects lambs and kids against pathogen CE (E(P)CK4) strain.