Abstract
Introduction. Type I collagen was examined using two types of atomic force microscopes (AFM) in a continuing effort to refine the process of obtaining molecular information from biological materials using scanning probe microscopy. Operating in air, a contact mode (Nanoscope II) and a non-contact mode AFM (Nanoscope III) were used to image collagen fibrils polymerized from pepsin-extracted type I bovine skin collagen adsorbed onto mica substrates. AFM is a practical method for high resolution examination of extracellular matrix material without the time consuming preparative techniques required for electron microscopy.Methods. For fibrillar collagen samples, Vitrogen 100 (Collagen Corporation, Palo Alto, CA) was prepared according to a modification of the procedure provided by Collagen Corp. for neutralized isotonic collagen gels. Monomeric collagen samples were prepared by diluting the vitrogen in 0.012M HCl.Images captured with the Nanoscope II AFM (Digital Instruments, Santa Barbara, CA) used a “J” scanner (horizontal range of 120 um).
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