Consumption of 5-hydroxymethylfurfural-rich dried fruits is associated with reduction in urinary excretion of 8-hydroxy-2′-deoxyguanosine: a randomized clinical trial

Abstract

5-Hydroxymethylfurfural is widely presented in foods and produced through the degradation of hexoses and the Maillard reaction. This study aimed to investigate any association between 5-HMF content of consumed dried fruits and urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG). The study consisted of 75 healthy volunteers. After an overnight fasting, two urine samples were collected before and 5 h after consumption of date, apricot, currant, plum and fruit bread, 100 g each. Twenty-five samples from frequently consumed types of dried fruits were analyzed for 5-HMF with HPLC. Urinary 5-hydroxymethyl-2-furoic acid (HMFA) and 8-OHdG were determined using HPLC and ELISA, respectively. The amount of 5-HMF in the tested samples varied from 314.8 ± 166.1 to 2496.3 ± 2301.2 mg/kg. The lowest and highest levels of 8-OHdG were 10.1 ± 1.5 µg/g creatinine for plum and 24.7 ± 6.6 µg/g creatinine for date. At the two time points, mean HMFA levels varied from 630.6 ± 114.5 to 2116.8 ± 600.3 and 286.7 ± 24.8 to 582.0 ± 119.2 (µg/g creatinine), respectively. There was a significant difference in HMFA amounts of the samples before and after consumption of apricot and plum (P 0.66, P < 0.01). It is concluded that consumption of 5-HMF-rich dried fruits is associated with a significant reduction in urinary excretion of 8-OHdG.