Abstract
Abstract The purpose of this study was to establish a suitable 2-dimensional polyacrylamide gel electrophoresis (2-DE) system for proteomics analysis in Brassica napus. Proteins of rapeseed variety 08127 were separated in gel brands (pH 4–7) using trichloroacetic extraction method under the improved isoelectric focusing (IEF) procedure. A clean 2-DE map was obtained, indicating that the system was effective and suitable for B. napus. This system was validated using young seedling, leaves, and stems from other varieties, and the results were similar to that of variety 08127. The proteomics profiling of seedlings in different ages of B. napus was then analyzed on the basis of this system. In the stems of 30-day-age seedlings, 20 up-regulated and 51 down-regulated proteins were found with differential expression more than 2 folds compared to their corresponding proteins in 15-day-age stems. A few differential protein spots were identified using mass spectrometry method, indicating the effectiveness of the 2-DE polyacrylamide gel electrophoresis system established in this study.
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