CONSTRUCTION OF THE INFECTIOUS MOLECULE OF BETA SATELLITE ASSOCIATED WITH AGERATUM YELLOW VEIN DISEASE OF AGERATUM CONYZOIDES

Abstract

Several dicot plant species are mostly infected by the vast variety of begomoviruses in different areas around the globe. Begomoviruses exhibit association with two satellites molecules, alphasatellites and betasatellites which are involved to cause severe viral infection. Whitefly plays a vital role as a vector in the transmission of virus from one plant to another. These viruses use weeds to cause infections when the major crop plants are absent in the field. Ageratum conyzoides is very important weed plant, infected with a unique virus complex. The study is intended to evaluate the satellite molecule diversity associated with yellow vein disease of A.conyzoides. Symptomatic leave samples of field grown ageratum plants were collected from different areas of Faisalabad. Betasatellites were extracted from infected ageratum plants.To amplify the beta satellite component clones, the rolling circular amplification was applied on extracted DNA-β isolated from infectious weed plants.The confirmation of DNA-β was done with the help of restriction by different suitable enzymes. The betasatellites were mainly focused in this study. The DNA-β was completely sequenced and infectious molecule was made.