Abstract
A supramolecular switch strategy that can reversibly “turn-on” and “turn-off” glutathione S-transferase (GST) is presented, which provides a proof-of-concept for a simple but efficient way to regulate the catalytic function of natural enzymes. This design is demonstrated by incorporating azobenzene/cyclodextrin-based supramolecular host–guest systems into the catalytic pocket of GST. The photoisomerization of trans- and cis-azobenzene leads to supramolecular complexation and dissociation of cyclodextrin, and thereby controls the enzymatic activity of GST by tuning substrate accessibility. This photoswitchable catalysis is reversible over multiple stimulus cycles. Furthermore, its capability is affected by the spatial size and binding affinity of different cyclodextrins, as well as the modification sites of azobenzene. The remote optical modulation method could offer great opportunities in the effort to create “smart” catalysts.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
