Construction of Refined Protein Interaction Network for Predicting Essential Proteins

Abstract

Identification of essential proteins based on protein interaction network (PIN) is a very important and hot topic in the post genome era. Up to now, a number of network-based essential protein discovery methods have been proposed. Generally, a static protein interaction network was constructed by using the protein-protein interactions obtained from different experiments or databases. Unfortunately, most of the network-based essential protein discovery methods are sensitive to the reliability of the constructed PIN. In this paper, we propose a new method for constructing refined PIN by using gene expression profiles and subcellular location information. The basic idea behind refining the PIN is that two proteins should have higher possibility to physically interact with each other if they appear together at the same subcellular location and are active together at least at a time point in the cell cycle. The original static PIN is denoted by S-PIN while the final PIN refined by our method is denoted by TS-PIN. To evaluate whether the constructed TS-PIN is more suitable to be used in the identification of essential proteins, 10 network-based essential protein discovery methods (DC, EC, SC, BC, CC, IC, LAC, NC, BN, and DMNC) are applied on it to identify essential proteins. A comparison of TS-PIN and two other networks: S-PIN and NF-APIN (a noise-filtered active PIN constructed by using gene expression data and S-PIN) is implemented on the prediction of essential proteins by using these ten network-based methods. The comparison results show that all of the 10 network-based methods achieve better results when being applied on TS-PIN than that being applied on S-PIN and NF-APIN.