Construction of rat vascular smooth muscle cell calcification model and VD3-induced vascular calcification in mice

Abstract

The methods of establishing the cell and animal calcification model were discussed. In vitro, the rat vascular smooth muscle cell strain A10 was cultured, the control group and the high phosphorus group were cultured for 12 days to stain, and the relationship between high phosphorus and vascular smooth muscle cell calcification was studied by reverse transcription PCR analysis. In vivo, the mice have injected with vitamin D3-induced calcification, 4 days of continuous injection, the growth status of mice was observed and the weight was recorded, and the blood vessels were made into paraffin sections for staining and observation. The model of Vitamin D3 induced vascular calcification in mice was constructed. This result showed that calcium salt deposition was found in the vascular smooth muscle cells of rats under high phosphorus condition, and the phenotype of vascular smooth muscle cells occurred. Vitamin D3 can induce vascular calcification in mice, and provide a reliable basis for the mechanism of Vitamin D3 atherosclerosis and clinical prevention.