Abstract
This study first reported a magnetic nanoflower biocatalyst of the core-shell magnetic composite microspheres with a hierarchical flower-like surface structure, which was consist of the organic component (horseradish peroxidase, HRP) and the inorganic component (Fe3O4@PMG-IDA-Cu2+) through self-assembly in the phosphate buffered saline (PBS) solution. The structure, pattern and crystallization of the magnetic nanoflowers were confirmed through a series of characterization. The optimized results of the magnetic nanoflowers formation conditions demonstrated that their hierarchical structure could effectively enhance the enzyme activity. The magnetic nanoflowers exhibited enhanced durability, stability and reusability through the study of enzymatic properties. The magnetic nanoflowers were applied to remove the bisphenol A (BPA) from water and the removal efficiency reached about 92.1%, meanwhile the enzymatic activity of the magnetic nanoflowers was achieved 183% enhancement in comparison with free HRP. In addition, the magnetic nanoflowers showed outstanding reusability and reproducibility, which would have potential application in biocatalysis.
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