Abstract
Late embriogenesis abundant (LEA) protein is one of the hot topics in plant stress physiology. In this study, an RNAi expression vector harboring MsLEA3-1 gene fragment from alfalfa ( Medicago sativa L.) was constructed. Two pairs of specific primers with different enzyme sites were designed based on the sequence of MsLEA3-1 (GenBank accession number EU665182). With the template of PMD-LEA plasmid constructed, positive-sense strand and antisense strand were obtained, which were separately inserted into the expression vector pART27. A hairpin structure in the RNAi vector pART-F-R was confirmed by the digestion of restriction enzymes. pART-F-R was transformed into tobacco via Agrobacterium-mediated transformation system, and 16 transgenic plants were obtained after PCR validation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
