Abstract
本研究通过三轮聚合酶链反应(PCR),用丙型肝炎病毒(HCV)非结构蛋白3(NS3)的辅助性T淋巴细胞(Th1)表位替换BALB/c小鼠MHC Ⅱ类分子恒定链(invariant chain,Ii)短肽(CLIP)编码基因,构建了替换CLIP片段的HCV-NS3 Th1内源性靶向基因疫苗,并在真核细胞中表达,为进一步研究HCV内源性靶向基因疫苗的功能奠定了基础。
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