Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis.

Palanisamy Bothammal,Karuppiah Ponmurugan,Naif Abdullah Al-Dhabi,Vellaisamy Vigneshwaran,Kalimuthusamy Natarajaseenivasan,Mohan Ganesh,Kumarasamy Anbarasu

doi:10.3389/fimmu.2021.764662

Palanisamy Bothammal, Karuppiah Ponmurugan + Show 5 more

Open Access

https://doi.org/10.3389/fimmu.2021.764662

Copy DOI

Abstract

Edwardsiella tarda is a severe aquaculture pathogen that can infect many hosts including humans, animals, and fish. Timely diagnosis and treatment are crucial for the control of edwardsiellosis in the aqua industry. By using rabbit polyclonal antibody, an expression gene library of virulent Edwardsiella tarda strain ED-BDU 1 isolated in south India was constructed and screened. The identified immune expressive proteins were characterized, and the corresponding coding sequences were cloned, expressed, and the purified recombinant proteins were used as antigens. The identified immunoreactive proteins namely HflC, HflK, and YhcI were studied for their immune protective potential in vivo by challenge experiments. The protective efficacy of HflC, HflK, and YhcI showed that the clearance of Edwardsiella from the host with ~ 60% survivability. Further, the immunoreactive proteins induce a strong immune response upon infection and elicit the significant production of IL-10, IFN-γ, Th1, and Th2 mediated mRNA expression and were therefore effective in vaccine production for edwardsiellosis.

Highlights

Aquaculture is a fast-growing fisheries sector globally, and India accounts second in culture fisheries production with an annual growth rate of over 7%
The advantage of building an immune expressive library is that prior knowledge of target antibodies is not required, eliminating the need to design primer oligonucleotides, and allowing the identification of new antigens
In this study, immunoscreening of the gene expression library was efficient at finding genes that encode antigenic proteins of E. tarda

Summary

Introduction

Aquaculture is a fast-growing fisheries sector globally, and India accounts second in culture fisheries production with an annual growth rate of over 7%. Aquaculture supplies dietary essentials for human consumption but provides opportunities for employment and income, especially in less economically developed rural areas. Sixty million people are directly engaged, part-time or full-time, in the primary production of fish, either by fishing or in aquaculture, supporting the livelihoods of 10-12% of the world population. It currently accounts for over 50% of global fish consumption. In India, carp varieties are highly cultivated, namely catla (Catla catla), rohu (Labeo rohita), and mrigal (Cirrhinus mrigala) [1].

Methods

Results

Conclusion