Abstract
Edwardsiella tarda is a severe aquaculture pathogen that can infect many hosts including humans, animals, and fish. Timely diagnosis and treatment are crucial for the control of edwardsiellosis in the aqua industry. By using rabbit polyclonal antibody, an expression gene library of virulent Edwardsiella tarda strain ED-BDU 1 isolated in south India was constructed and screened. The identified immune expressive proteins were characterized, and the corresponding coding sequences were cloned, expressed, and the purified recombinant proteins were used as antigens. The identified immunoreactive proteins namely HflC, HflK, and YhcI were studied for their immune protective potential in vivo by challenge experiments. The protective efficacy of HflC, HflK, and YhcI showed that the clearance of Edwardsiella from the host with ~ 60% survivability. Further, the immunoreactive proteins induce a strong immune response upon infection and elicit the significant production of IL-10, IFN-γ, Th1, and Th2 mediated mRNA expression and were therefore effective in vaccine production for edwardsiellosis.
Highlights
Aquaculture is a fast-growing fisheries sector globally, and India accounts second in culture fisheries production with an annual growth rate of over 7%
The advantage of building an immune expressive library is that prior knowledge of target antibodies is not required, eliminating the need to design primer oligonucleotides, and allowing the identification of new antigens
In this study, immunoscreening of the gene expression library was efficient at finding genes that encode antigenic proteins of E. tarda
Summary
Aquaculture is a fast-growing fisheries sector globally, and India accounts second in culture fisheries production with an annual growth rate of over 7%. Aquaculture supplies dietary essentials for human consumption but provides opportunities for employment and income, especially in less economically developed rural areas. Sixty million people are directly engaged, part-time or full-time, in the primary production of fish, either by fishing or in aquaculture, supporting the livelihoods of 10-12% of the world population. It currently accounts for over 50% of global fish consumption. In India, carp varieties are highly cultivated, namely catla (Catla catla), rohu (Labeo rohita), and mrigal (Cirrhinus mrigala) [1].
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.