Abstract
In this work, two new turnip mosaic virus (TuMV) strains (Canola-12 and Canola-14) overcoming resistance in canola (Brassica napus) were isolated from a B. napus sample that showed typical TuMV-like symptoms and was collected in the city of Gimcheon, South Korea, in 2020. The complete genome sequence was determined and an infectious clone was made for each isolate. Phylogenetic analysis indicated that the strains isolated from canola belonged to the World-B group. Both infectious clones, which used 35S and T7 promoters to drive expression, induced systemic symptoms in Nicotiana benthamiana and B. napus. To our knowledge, this is the first report of TuMV infecting B. napus in South Korea.
Highlights
Brassica napus, belonging to the genus Brassica, in the family Brassicaceae, is extensively utilized as vegetable oil, biodiesel, and livestock feed around the world [1]
An increasing number of studies have reported new Turnip mosaic virus (TuMV) isolates occurring in China, South Korea and Japan, which reflects the prevalence of TuMV in east Asia [15–17]
A phylogenetic tree was constructed using the Maximum-likelihood method with 1000 bootstrap replicates in MEGA and based on the complete genomic sequences of TuMV isolates from NCBI, including 25 isolates previously identified in South Korea [16, 17, 21]
Summary
Brassica napus (canola or oilseed rape), belonging to the genus Brassica, in the family Brassicaceae, is extensively utilized as vegetable oil, biodiesel, and livestock feed around the world [1]. Symptoms caused by TuMV in Brassica crops depend on virus isolate, host plant and environmental conditions, mainly including systemic vein clearing, mosaic, necrosis, and plant stunting [3]. An increasing number of studies have reported new TuMV isolates occurring in China, South Korea and Japan, which reflects the prevalence of TuMV in east Asia [15–17].
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