Construction of expression vector of CryIA(c) gene and its transformation in sugarcane

Abstract

A plant expression vector, pGCryIA(c), was constructed by linking CryIA(c) gene which has UBI as promoter and NOS as terminator into pGreenII 0229. The size of pGCryIA(c) is 8,602 bp with bar (phosphinothricin acetyl transferase) gene as screening marker gene by using herbicide phosphinothricin (PPT) or Basta selection. The embryonic callus of GT94-119 was transformed with the plasmid of pUBCG0229 by bombardment. 203 regenerated plants were obtained after screening with PPT or Basta. Two transformation events were proved sucessful by PCR, PCR products sequencing and Dot-Southern blotting.