Abstract
• ERGO/GCE showed obvious catalysis to voltammetric behaviors of all five active components in terms of peak current and potential. • ERGO film electrode was used for HPLC with amperometric detection. • The possible electrode processes of five components at the surface of ERGO/GCE have been estimated herein. • This method coupled with HPLC could be applied to analysis of extractive from O. fragrans fruits and the results is competitive compared to those by HPLC-MS. Neonuezhenide, nuezhenide, acteoside, isoacteoside and salidroside are common phenylethanoid glycosides in Osmanthus fragrans ( O. fragrans ) fruits and have various physiological and pharmacological activities. The electrochemical determination of the five components in O. fragrans fruits was researched at electrochemically reduced graphene oxide film modified glassy carbon electrode (ERGO/GCE). ERGO film was prepared by one-step deposition and showed a honeycombed network structure and provided good conductivity, controllability and stability. Compared with bare GCE or chemically reduced graphene oxide film modified electrode (RGO/GCE), the voltammetric behaviours of all five active components were improved at ERGO/GCE in terms of peak current and potential due to excellent electrical properties of ERGO. The numbers of protons and electrons transferred in electrochemical redox reaction were calculated and the possible electrode processes of five components have been estimated herein. The number of electrons involved was equal to that of protons transferred in the electrode processes for each compound and it was 2 for neonuezhenide, 1 for nuezhenide, 4 for acteoside, 4 for isoacteoside, and 1 for salidroside, respectively. Finally, a simple electrochemical method was developed for determination of the five components. This method has wide linear range with low detection limit of 3 μM for neonuezhenide, 6 μM for nuezhenide, 4 μM for acteoside, 3 μM for isoacteoside, and 3 μM for salidroside, respectively. Moreover, this method could be coupled with high performance liquid chromatography (HPLC) and applied to determination of the five active components in extractive from O. fragrans fruits. Compared with high performance liquid chromatography-mass spectrometry (HPLC-MS), this method would be competitive and alternative for the analysis of active components in real samples.
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