Year-end Sale % OFF 
Abstract
Various methods have been used to reconstruct the penis. The objective of this study was to investigate the feasibility of constructing engineered corpus cavernosum with primary mesenchymal stem cells (MSCs) in a rabbit model in vitro. Acellular corporal matrices (ACMs) were obtained from adult rabbit penile tissues through an established decellularization procedure. MSCs were separated, purified, and then seeded on ACMs to construct engineered corpus cavernosum. The seeded ACMs were subsequently cultured in an incubator for 14 days. Histological analyses showed that MSCs seeded on the ACMs had proliferated and were well distributed. Detection of CD31, vWF, smooth muscle actin (SMA), and myosin protein as well as vWF and myosin mRNA revealed that the MSCs had differentiated into endothelial cells and smooth muscle cells. In addition, cell morphology of the engineered corpus cavernosum was directly observed by transmission electron microscopy. This study demonstrated that engineered corpus cavernosum could be successfully constructed using primary MSCs in vitro. This technology represents another step towards developing engineered corpus cavernosum in vitro.
Highlights
Various methods have been used to reconstruct the penis
mesenchymal stem cells (MSCs) were successfully isolated from bone marrow by the Percoll density gradient centrifugation method, and the MSCs were adherent
The results showed that vWF (Fig. 4F) and myosin (Fig. 4G) mRNA expressed in normal tissues and in the engineered corpus cavernosum but not in MSCs
Summary
The objective of this study was to investigate the feasibility of constructing engineered corpus cavernosum with primary mesenchymal stem cells (MSCs) in a rabbit model in vitro. This study demonstrated that engineered corpus cavernosum could be successfully constructed using primary MSCs in vitro. Previous researchers have seeded differentiated cells, including smooth muscle cells, endothelial cells[14,15,16] and umbilical artery smooth muscle cells[17], on acellular corporal matrices (ACMs) to reconstitute functional corpus cavernosum. We sought to evaluate the use of primary MSCs as seeding cells to construct engineered corpus cavernosum in vitro. We analysed the differentiation of MSCs seeded on ACMs. The findings of this study represent another step towards the construction of engineered corpus cavernosum in vitro
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
