Abstract
The assembly of polyelectrolytes and biomacromolecules (proteins, DNA) has attracted great attention in several fields related to medicine, molecular biology, and clinical chemistry. Here, a quick and easy multicomponent self-assembly is used to unite two species with the same charge to form an electroactive polyamine-enzyme assembly. Phosphate ions are added as a crosslinker agent to create a stable structure. Osmium-derivatized polyallylamine is combined with horseradish peroxidase (pI = 8.6) or PQQ-glucose dehydrogenase (pI = 9.5) on graphite electrodes. The assemblies are built by layer-by-layer or one-pot deposition. In both cases, the electrocatalytical signals observed are comparable to the state-of-the-art results. The proposed construction method represents a new alternative for the assembly of polyamines with proteins independently of their charge.
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