Construction of dual receptors imprinted polymers by precise control of sequential assembly efficiency in confined sites to enhance specific separation of adenosine 5′-monophosphate

Abstract

Sequential assembly strategies are employed to incorporate various monomers, thereby enhancing the specific binding capability of imprinting sites towards the target molecule and ultimately improving the selectivity of molecular imprinted polymers (MIPs). However, the effectiveness of sequential assembly in improving discriminative separation requires further investigation. For the specific functional groups present in AMP molecules, the utilization of confining metal ion sites guided by metal–organic frameworks (MOFs) material can effectively mitigate disordered competition, enhance the efficiency of imprinted assembly, and facilitate the controlled growth of imprinted sites. In this study, we have successfully achieved precise control over the efficiency of sequential assembly in hydrophilic Zr-MOFs nanosheets in order to fabricate dual receptor surface molecularly imprinted sorbents (D-MIPs) for selectively separating AMP. The hydrophilic Zr-MOFs nanosheets (D-PABA) possess a significantly high surface area of approximately 282.52 m2 g−1. The water contact angle of Zr-BTB-FA nanosheets was measured to be 32°, while that of D-MIPs was 25°. The hydrophilic surface properties of materials promote the dispersion of the adsorbent in aqueous solutions and enhance the rapid mass transfer of water-soluble target molecules. Additionally, the half-equilibrium time (t1/2) was found to be 0.578 h. The D-MIPs demonstrate a significant adsorption capacity (286.7 μmol g−1 at 298 K) for AMP compared to other nucleoside analogues, such as dC, dG, dA, ADP and ATP. Additionally, an imprinting factor (IF) of 2.764 was observed for D-MIPs compared to their non-imprinted counterpart. In addition, the selective extraction of 97 % AMP from the spiked diluted biofermentation broth sample can be achieved using D-MIPs. This finding highlights the potential of D-MIPs as an efficient solution for the selective capture of AMP from biological samples.