Abstract
BackgroundDihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory.ResultTo produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain.ConclusionTo our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11.
Highlights
Polyunsaturated fatty acids (PUFAs) are attracting significant interest because they have important roles in human health and nutrition [1]
To our knowledge, this is the first report describing the overexpression of delta-6 elongase (D6E) (GLELO) gene in M. circinelloides to construct dihomogamma linolenic acid (DGLA) producing cell factory
A new scope for further research has been established by this work for improved production of DGLA in this fungus, in its high lipid-producing strain, WJ11
Summary
Polyunsaturated fatty acids (PUFAs) are attracting significant interest because they have important roles in human health and nutrition [1]. Mammals, including humans, can synthesize saturated fatty acids (SAFA) and monounsaturated fatty acids (MUFA) but cannot synthesize de novo omega-6 or omega-3 polyunsaturated fatty acids (PUFA) which are linoleic acid (LA, 18:2, n-6) and α-linolenic acid (ALA, 18:3, n-3) respectively These PUFA are essential fatty acids (EFAs) for human health and must be obtained from the diet [1, 2]. LA and ALA are metabolised to Docosapentaenoic acid (DPA, 22:5, n-6) and Docosahexaenoic acid (DHA, 22:5, n-3) respectively in a series of reactions catalyzed by the same sets of enzymes [3] Several diseases such as obesity, hypertension, diabetes mellitus, coronary heart disease, schizophrenia, Alzheimer’s disease, atherosclerosis, and cancer can alter the metabolism of essential fatty acids (EFAs). We have overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory
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