Construction of cytoskeleton modulated by phosphatase and tensin homolog deleted on chromosome ten/mammalian target of rapamycin during the differentiation of bone marrow stem cell-derived nerve cells

Abstract

Objective To observe the construction of cytoskeleton modulated by phosphatase and tensin homolog deleted on chromosome ten (PTEN)/mammalian target of rapamycin (mTOR) during the nerve cell differentiation by human bone marrow stem cells in vitro,and to analyze the implication.Methods Adult bone marrow cells were isolated and induced into nerve-like cells by some cytokines in vitro after 2 weeks.The mRNA expression of mTOR which is the downstream molecule of PTEN was compared between un-treated and treated by the BPV (a type of PTEN inhibitor) using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).The construction patterns of cytoskeleton were indicated by Phalloidin-fluoresceine isothiocyanate (FITC),and observed by the fluorescence microscopy.Results Neuron specific enolase (NSE) and β-Ⅲ tubulin were expressed in these cells after the induction by adult human bone marrow stem cells.nestin,the marker of neural progenitors was expressed increasingly at the beginning,and decreasingly subsequently.The mRNA expression level of mTOR was increased and the lenth of the neurites was from (4.58 ± 1.21) μm to (9.09 ±2.68) μm after the cells were treated by BPV (P ＜ 0.05).Conclusion When the nerve cells derived from bone marrow stem cells obtain the mature differentiation,PTEN/mTOR may possess the the ability of cytoskeleton construction modulation in the neuritis of nerve cells in vitro. Key words: Nerve cell; Cell differentiation; Phosphatase and tensin homolog deleted in chromosome 10; Mammalian target of rapamycin; Cytoskeleton