Construction of cytogenetic map of Gossypium herbaceum chromosome 1 and its integration with genetic maps.

Xinglei Cui,Chunying Wang,Xiaoyan Cai,Zhongli Zhou,Yuling Liu,Yanyan Zhao,Yuhong Wang,Xingxing Wang,Renhai Peng,Fang Liu,Kunbo Wang,Fei Meng

doi:10.1186/s13039-015-0106-y

Xinglei Cui, Chunying Wang + Show 10 more

Open Access

https://doi.org/10.1186/s13039-015-0106-y

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Abstract

BackgroundCytogenetic map can provide not only information of the genome structure, but also can build a solid foundation for genetic research. With the developments of molecular and cytogenetic studies in cotton (Gossypium), the construction of cytogenetic map is becoming more and more imperative.ResultsA cytogenetic map of chromosome 1 (A101) of Gossypium herbaceum (A1) which includes 10 bacterial artificial chromosome (BAC) clones was constructed by using fluorescent in situ hybridization (FISH). Meanwhile, comparison and analysis were made for the cytogenetic map of chromosome 1 (A101) of G. herbaceum with four genetic linkage maps of chromosome 1 (Ah01) of G. hirsutum ((AD)1) and one genetic linkage map of chromosome 1 of (A101) G. arboreum (A2). The 10 BAC clones were also used to be localized on G. raimondii (D5) chromosome 1 (D501), and 2 of them showed clear unique hybridized signals. Furthermore, these 2 BAC clones were also shown localized on chromosome 1 of both A sub-genome and D sub-genome of G. hirsutum.ConclusionThe comparison of the cytogenetic map with genetic linkage maps showed that most of the identified marker-tagged BAC clones appearing same orders in different maps except three markers showing different positions, which might indicate chromosomal segmental rearrangements. The positions of the 2 BAC clones which were localized on Ah01 and Dh01 chromosomes were almost the same as that on A101 and D501 chromosomes. The corresponding anchored SSR markers of these 2 BAC clones were firstly found to be localized on chromosome D501 (Dh01) as they were not seen mapped like this in any genetic map reported.

Highlights

Cytogenetic map can provide information of the genome structure, and can build a solid foundation for genetic research
The chromosome-specific bacterial artificial chromosome (BAC) clone 52D06 was used to identify chromosome 1(A101) of G. herbaceum [41,42], and all positive BAC clones of 16 SSR markers were selected for fluorescent in situ hybridization (FISH) mapping
The cytogenetic map including the ten BAC clones are all anchored by SSR markers

Summary

Introduction

Cytogenetic map can provide information of the genome structure, and can build a solid foundation for genetic research. With the developments of molecular and cytogenetic studies in cotton (Gossypium), the construction of cytogenetic map is becoming more and more imperative. The genus Gossypium, which comprises of 52 species had been classified into 8 diploid (2n = 2× = 26) genomes, i.e. A, B, C, D, E, F, G, and K, and as well one allotetraploid (2n = 4× = 52) genome, i.e. AD [3,4]. In the early stages of the genus evolution, A genome diploids. This proves that cotton (Gossypium spp) is an important economic plant, and an excellent system for study on genomic organization, genome-size variation, genome evolution and polyploidization in plants. Due to its importance in scientific research, sequencing the cotton genome and carrying out genomic research are highly necessary

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