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Abstract
We used the minitransposon Tn hlyA s [Gentschev, I., Maier, G., Kranig, A. and Goebel, W. (1996) Mol. Gen. Genet. 252, 266–274] for random insertion of the secretion signal (HlyA s) of Escherichia coli hemolysin (HlyA) into chromosomal genes. Four mini-Tn hlyA s derivatives bearing the gltA (citrate synthase), deoC (2 deoxyribose-5 phosphate aldolase), tig (trigger factor) genes and an unknown ORF fused to hlyA s were identified and characterized. Our data suggest that Tn hlyA s-generated hemolysin fusion proteins are secreted efficiently by the HlyB/HlyD/TolC hemolysin secretion machinery and that this can be useful for studies of gene expression or function.
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