Abstract
This work reports a study on the development of a sensitive immunosensor for the assay of actin, which is fabricated using sensing material chitosan-Zn nanoparticles (NPs) and anti-actin modified on glassy carbon electrode respectively. The prepared materials were characterized using transmission electron microscope (TEM), fourier transform infrared spectra (FTIR), X-ray diffraction (XRD) spectra, and circular dichroism (CD) techniques. Meanwhile, the electrochemical properties were studied by linear sweep voltammetric (LSV), electrochemical impedance spectra (EIS), and differential pulse voltammetry (DPV). According to the experiments, under the optimum conditions, the linear fitting equation was I (μA) = −17.31 + 78.97c (R2 = 0.9948). The linear range was from 0.0001 to 0.1 mg/mL and the detection limit (LOD, S/N = 3) was 21.52 ng/mL. The interference studies were also performed for checking the sensors’ selectivity to actin. With better properties of the chitosan-Zn NPs, the modified electrode is considered as a better candidate than Western blot or immunohistochemical method for real-time usability. The detection limit reported is the lowest till date and this method provides a new approach for quality evaluation.
Highlights
Actin is the most abundant protein in most eukaryotic cells
1A, it was demonstrated thatthat chitosan formed a template and Zn was firmly and
A rapid, accurate and convenient approach was described for actin analysis based on the construction of chitosan-Zn NPs and anti-actin based electrochemical biosensing platform
Summary
Actin is the most abundant protein in most eukaryotic cells. It participates in many important physiological processes including muscle contraction, cell motility, cell division, and so on. The actin cytoskeleton, as an extremely dynamic and highly ordered cell component, underpins abundant cellular processes and is vital for tumor proliferation and metastasis [1,2]. The studies of actin are mainly in cytoskeleton structure and rearrangement [6,7,8,9]. Kim et al designed a fluorescently labeled method for direct monitoring of actin utilizing a modified protein transduction tag from the HIV TAT sequence [10]. Shimozawa et al used an actin filament labeled with either
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