Abstract
Here we applied a magnetic force-based tissue engineering technique to cardiac tissue fabrication. A mixture of extracellular matrix precursor and cardiomyocytes labeled with magnetic nanoparticles was added into a well containing a central polycarbonate cylinder. With the use of a magnet, the cells were attracted to the bottom of the well and allowed to form a cell layer. During cultivation, the cell layer shrank towards the cylinder, leading to the formation of a ring-shaped tissue that possessed a multilayered cell structure and contractile properties. These results indicate that magnetic tissue fabrication is a promising approach for cardiac tissue engineering.
Highlights
It is well known that the human heart has a limited capacity for regeneration after myocardial infarction
In order to magnetically label cells, Magnetite cationic liposomes (MCLs) were added to cells that had adhered onto the culture surfaces
Cardiomyocytes were labeled with MCLs by incubating the cells with MCLs in suspension to avoid cell damage at the cell harvest using a digestive enzyme
Summary
It is well known that the human heart has a limited capacity for regeneration after myocardial infarction. Heart transplantation is a therapeutic option, it is restricted due to a lack of suitable donor organs. 2010, 11 reconstructed using tissue engineering techniques provide a new muscle material for tissue replacement therapy [1,2]. Sci. 2010, 11 reconstructed using tissue engineering techniques provide a new muscle material for tissue replacement therapy [1,2] Such artificial tissue constructs can serve as important tools for in vitro cardiac tissue models to study cardiac developmental biology and to screen pharmacological agents for heart disease treatments. In addition to applications in the biomedical field, various bio-actuators, in which kinetic forces generated by engineered cardiac tissues are utilized as driving forces, have been developed [3,4,5]. Significant efforts are currently focused on developing three-dimensional (3D)
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