Construction of biomimetic vascular network applied to a tissue-engineered liver scaffold with computer assisted

Abstract

Objective To construct biomimetic vascular network of tissue engineering liver scaffold.Methods Using 64 row CT to collect normal liver blood vessels image,then transform the CT images into 4 cm× 4 cm vascular fault plane coordinate chart,according to liver vessels different section plane coordinates,laser prepare biomimetic vascular network on the electrospun Poly (EC-CL) scaffolds.The scanning election microscopy (SEM) tested the bionic vascular network.each of the liver tissue engineering scaffolds will punch in the order attached together using the matrigel adhesive.Using green fluorescent protein (GFP)-adenovirus marked euro-collins solution (ECs),then implanted ECs into bionic blood vessels in the bionic network.Cultured the bionic liver vessel network at 37 ℃,5％ CO2 incubator,after 3 weeks culture,tested the formation of blood vessels with fluorescence microscope.Results After GFP-adenovirus transfected mesenchymal stem cells (MSCs) as MOI =0,5,25,50,100 a week,the group of MOI =100 has the highest transfected rate (82.13 ± 3.65) ％.Cells proliferation test shows no statistical significance in all 5 groups (P ＞ 0.05).Endothelial cells migration test still has no statistical significance (P＞0.05).the 5 groups migration date:(32.54 ± 1.14),(28.13±1.43),(28.89± 1.01),(27.28 ± 0.52) and (33.14 ± 0.98)/HP respectively.After laser drilling,scanning electron microscopy (sem) shows the bionic vascular edge smooth.After 3 weeks culture,immune fluorescent detected visible wall endothelial layer formation.Conclusion Helped to promote metabolism functional tissue engineering viscera research progress. Key words: Bionic liver; Vascular network ; Adenovirus