CONSTRUCTION OF BIOMIMETIC POLYMER SURFACE FOR ENDOTHELIAL CELL SELECTIVITY

Abstract

This paper aimed to describe a bioactive polymer biointerface for endothelial cells specifically recognition.In particular,biomembrane mimetic polymers with a phospholipid group were designed.A phospholipid polymer-poly[2-methacryloyloxyethyl phosphorylcholine(MPC)-co-n-stearyl methacrylate(SMA)-co-p-nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate(MEONP)](PMSN) was synthesized using AIBN as an initiator and coated on substrate(PET) by solvent evaporation.MPC unit could reduce nonspecific adsorption such as platelet,protein and cells.MEONP unit contains active ester groups on the side chains for immobilization of antibody or peptide sch as REDV.The peptide sequence Arg-Glu-Asp-Val(REDV) was known to bind preferentially to endothelial cells.As a result,MEONP units could conjugate REDV and enhance the specific endothelial cells adsorption.HNMR,UV and FTIR spectrum were used to characterize the composition of polymer and immobilization of REDV peptide plasma recaleification time(PRT) was detected to show the hemocompatibility of polymer coating.Cell adhesion and proliferation experiments showed excellent specific recognition of endothelia cells on biomimetic coatings.Many endothelial cells were observed and the number of endothelial cells adhering to the PMSN polymer surfaces increased but cell adhesion was markedly reduced on poly(MPC-co-SMA)(PMS).For smooth muscle cells(SMC),many adherent cells were observed and well spread on PET but little on PMS and PMSN by the reason of nonspecific adhesion of MPC for smooth muscle cells.In conclusion,MEONP units and MPC units of PMSN are important for supporting ECs and for reducing SMCs.The PMSN-coated surface was suitable for biorecognition and preservation of cell function.The biomembrane mimetic phospholipid polymer produced an excellent prospect in cardiovascular stent coating.