Abstract
目的 用自体富血小板血浆(PRP)、骨髓基质干细胞(BMSCs)和纤维蛋白胶(FG)构建可注射型组织工程骨并从放射学方面评价其修复自体兔桡骨缺损的效果. 方法 36只新西兰兔随机分为 A、B、C三组,每组 12只.所有动物术前 1周抽取耳背中央动脉血提取 PRP. A组于术前 1～ 2个月抽取双髂骨处骨髓并培养出 BMSCs,在体外与 FG及自体 PRP构建成可注型组织工程骨,植入自体桡骨 1.5cm节段性骨缺损,为实验组. B、C两组分别植入 PRP+ FG、FG于同样骨缺损处为对照组.另取 4只桡骨同样部位和大小骨缺损旷置作为空白组.术后 4、8、12周时摄前臂正位 X线片.用放射影像学评分和图像分析 X线阻射影比较骨缺损的修复情况. 结果 A、B两组骨缺损放射影像学评分以及新生骨痂的密度在 4、8、12周均优于 C组 (P 0.05),但在新生骨痂密度上, A >B >C(P< 0.05).空白组术后 16周骨缺损无愈合. 结论 PRP对骨缺损愈合有明显促进作用,复合 PRP的可注射型材料与 BMSCs构建组织工程骨可修复兔桡骨节段性骨缺损。
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