Abstract
An infectious full-length cDNA clone of Chrysanthemum virus B (CVB, genus Carlavirus), was constructed. Four cDNA fragments covering the whole genome of CVB-S were cloned between the Cauliflower mosaic virus 35S promoter and the nopaline synthase (NOS) terminator. Chrysanthemum and garland chrysanthemum were inoculated with the constructed plasmid, named pCVB, using a gene gun system. As is the case in wild-type, CVB-infected plants, no visible symptoms were observed on plants inoculated with pCVB; however, western blotting and electron microscopy indicated the presence of the progeny virus of pCVB. pCVB could be a useful tool for analyzing the functions of carlaviral proteins.
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