Abstract
Using a specialized transducing λ phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The Δ( umuDC)595:: cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuD,C proteins, which are normally required for inducible mutagenesis, are not essential for cell survival
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