Construction of a red fluorescent shuttle vector controlled by recA operon promoter of Streptococcus mutans

Abstract

To construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans. The promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred. pLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed. The recombination plasmid pLRred can be used in the further research of the expression of cariogenic virulence factor gene by Streptococcus mutans in biofilm.