Abstract
A red emission BODIPY-based lysosome-targeted viscosity probe (Lys-VBOD) was synthesized for monitoring lysosomal viscosity in live cells. The increase of viscosity can restrict the twisting of double bond between indole and BODIPY fluorophore, also restrict the rotating of single bond between quinoline and BODIPY, which lead to the enhancement of fluorescence intensity in high viscosity environment. The changes of fluorescence intensity exhibit a good linear relationship with viscosity values. Besides, Lys-VBOD is quite stable in various pH solvents with given viscosity, suggesting that Lys-VBOD is suitable for applying in a wide pH range. Moreover, Lys-VBOD can visualize the changes of lysosomal viscosity in live cells under the stimulus of dexamethasone. BSA titration experiment demonstrated that Lys-VBOD is stable in the presence of macromolecules and proteins. Notably, the fluorescence lifetime of Lys-VBOD in large viscosity solvents is extremely high. These results indicate that Lys-VBOD is an excellent tool for detecing viscosity changes in vitro and live cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
