Construction of a paper-based electrochemical biosensing platform for rapid and accurate detection of adenosine triphosphate (ATP)

Abstract

In this paper, a simple but efficient electrochemical biosensing platform was developed for ATP assay based on the preparation of paper-based working electrodes. In contrast to the electrochemical response of commercial glassy carbon electrode (GCE), improved voltammetric signals were achieved at the paper-based electrode. In particular, the surface contamination arising from oxidation product, a prominent challenge for ATP detection, was effectively eliminated by the paper-based system, endowing reliable analysis with high reproducibility. Moreover, the practical application value of the system was verified by assay of ATP in human serums, cancer cells, and normal cells with satisfactory results. Compared with the detection performances of traditional strategies, the advantages involved in the system were exhibited to be rapid, accurate, convenient, and inexpensive, which confer the proposed sensing platform promising for applications in public health as well as the fundamental research of molecular biology.