Construction of a microbial consortium used for solid-state fermentation on rice chaff

Abstract

Trichoderma reesei 3.3711 and Aspergillus niger 3.316 were co-cultured with Saccharomyces cerevisiaes AS2.399 in solid substrate fermentation on rice chaff for its intenerate. Pretreatment of rice chaff was supplemented with appropriate wheat bran, inorganic N-sources and some metal compounds, and fermented at 100% moisture content, pH 5.5 and 30 °C. Under the same fermentation conditions, mixed culture of three microorganisms had higher ratio of cellulose degradation (RCD) than mixed culture of T. reesei 3.3711 and A. niger 3.316, and mixed culture of two microorganisms had higher RCD than single microorganisms of T. reesei 3.3711 and A. niger 3.316, respectively. But the same law was not found in filter paper enzyme activity (FPA). It was found that mixed culture of three microorganisms had 4.82% higher RCD than two strains culture, and two strains culture had 12.68% higher than T. reesei 3.3711 which had 11.14% higher than A. niger 3.316. According to the microbial consortium this article constructed is invaluable for exploiting cellulose resources.