Abstract
A novel and simple optical biosensor to detect triglycerides (TGs) has been successfully constructed by using pectin hydrogel membrane as the indicator pH and chromoionophore ETH 5294 (CI), with lipase as the catalyst. The enzymatic working system against TGs releasing H+ ions will affect the color absorbance of CI. The characterization results show that a TG biosensor has the optimum condition and sensitivity at the phosphate buffer concentration of 50 mM, pH 7, and enzyme loading of 60 μg. The biosensor works at the tripalmitin (TP) concentration range of 100–400 mg/dL. With the sensitivity of 0.001 (∆A/(mg/dL)), the biosensor response reaches stability after five minutes, and the limit of detection (LOD) of the TG optical biosensor is 15 mg/dL. Relative standard deviation (RSD) in a reproducibility test was 2.5%, with a 15-day lifespan.
Highlights
A triglyceride (TG), known as a type of biolipid, is a triester of glycerol bound to three fatty acids, with varying saturation degrees between 0 and 6 [1]
Pectin hydrogel membrane is made with a CaCl2 crosslinker
The obtained hydrogel that the pectin membrane is homogenous, giving it a smooth surface, which is important for the absorbance membrane is colorless, and morphological observation with scanning electron microscopy (SEM)
Summary
A triglyceride (TG), known as a type of biolipid, is a triester of glycerol bound to three fatty acids, with varying saturation degrees between 0 and 6 [1]. Elevated TG concentrations in the body may result in cardiovascular and coronary heart diseases [4,5,6,7]. Due to this health concern, more effective ways to determine TG concentrations in food, beverage, and cosmetic products must be studied. With the rapid advancement of science and technology, cutting-edge equipment has been developed for the detection of TGs. At present, with the rapid advancement of science and technology, cutting-edge equipment has been developed for the detection of TGs These include colorimetry [8,9], spectrophotometry [10,11,12,13,14], chromatography [15,16], fluorometry [17], titrimetry [18], nuclear magnetic resonance [19], and enzymatic colorimetry [20].
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