Abstract
Monoclonal antibodies generated by modern biotechnology are useful as therapeutic agents for many kinds of diseases. Laboratory mice are a ready source for generating high-affinity and specificity monoclonal antibodies. However, the limitation of rodent antibodies as therapeutic molecules forces many biotechnology groups to explore new technologies to generate safer and more efficacious human antibodies, which includes the construction of transgenic mice encoding human sequences. We report here that human umbilical cord blood cells, containing some stem cells, were introduced into mouse fetal peritoneal cavities to generate humanized chimeric mice. The expressions of human immunoglobulin genes were detected in peripheral blood of adult chimeric mice. Our study demonstrates successful production of humanized chimeric mice by fetal peritoneal cavity injection with human umbilical cord blood cells. The chimeras are likely to be a valuable tool for obtaining therapeutic human antibodies.
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