Abstract
High-density genetic linkage maps of half-smooth tongue sole were developed with 1007 microsatellite markers, two SCAR markers and an F1 family containing 94. The female map was composed of 828 markers in 21 linkage groups, covering a total of 1447.3 cM, with an average interval 1.83 cM between markers. The male map consisted of 794 markers in 21 linkage groups, spanning 1497.5 cM, with an average interval of 1.96 cM. The female and male maps had 812 and 785 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1527.7 cM for the females and 1582.1 cM for the males. Based on estimations of the map lengths, the female and male maps covered 94.74 and 94.65% of the genome, respectively. The consensus map was composed of 1007 microsatellite markers and two SCAR markers in 21 linkage groups, covering a total of 1624 cM with an average interval of 1.67 cM. Furthermore, 159 sex-linked SSR markers were identified. Five sex-linked microsatellite markers were confirmed in their association with sex in a large number of individuals selected from different families. These sex-linked markers were mapped on the female map LG1f with zero recombination. Two QTLs that were identified for body weight, designated as We-1 and We-2, accounted for 26.39% and 10.60% of the phenotypic variation. Two QTLs for body width, designated Wi-1 and Wi-2, were mapped in LG4f and accounted for 14.33% and 12.83% of the phenotypic variation, respectively. Seven sex-related loci were mapped in LG1f, LG14f and LG1m by CIM, accounting for 12.5–25.2% of the trait variation. The results should prove to be very useful for improving growth traits using molecular MAS.
Highlights
Genetic linkage maps have become important tools in many areas of genetic research
The microsatellite locus derived from the sequence of the SOX9 genes in half-smooth tongue sole was linked to LG18
Linkage analysis and map construction using molecular markers is more complicated in full-sib families of out-breeding species than in progenies derived from homozygous parents
Summary
Genetic linkage maps have become important tools in many areas of genetic research. To perform a linkage study, it is necessary to genotype and map large numbers of the available genetic markers on mapping families. Genetic linkage maps based on microsatellite markers have been generated for economically important marine species, such as salmon [1], tilapia [2], European sea bass [3], rainbow trout [4], sea bream [5], Barramundi [6], catfish [7], grass carp [8], Japanese flounder [9] and Asian sea bass [10]. QTL for growth, disease resistance and stress response have been mapped in only a few species, such as Asian sea bass [10], rainbow trout [13], tilapia [14], salmon [15], Japanese flounder [16], guppy [17] and European seabass [18]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
