Abstract
BackgroundThe genetics and molecular biology of sesame has only recently begun to be studied even though sesame is an important oil seed crop. A high-density genetic map for sesame has not been published yet due to a lack of sufficient molecular markers. Specific length amplified fragment sequencing (SLAF-seq) is a recently developed high-resolution strategy for large-scale de novo SNP discovery and genotyping. SLAF-seq was employed in this study to obtain sufficient markers to construct a high-density genetic map for sesame.ResultsIn total, 28.21 Gb of data containing 201,488,285 pair-end reads was obtained after sequencing. The average coverage for each SLAF marker was 23.48-fold in the male parent, 23.38-fold in the female parent, and 14.46-fold average in each F2 individual. In total, 71,793 high-quality SLAFs were detected of which 3,673 SLAFs were polymorphic and 1,272 of the polymorphic markers met the requirements for use in the construction of a genetic map. The final map included 1,233 markers on the 15 linkage groups (LGs) and was 1,474.87 cM in length with an average distance of 1.20 cM between adjacent markers. To our knowledge, this map is the densest genetic linkage map to date for sesame. 'SNP_only’ markers accounted for 87.51% of the markers on the map. A total of 205 markers on the map showed significant (P < 0.05) segregation distortion.ConclusionsWe report here the first high-density genetic map for sesame. The map was constructed using an F2 population and the SLAF-seq approach, which allowed the efficient development of a large number of polymorphic markers in a short time. Results of this study will not only provide a platform for gene/QTL fine mapping, map-based gene isolation, and molecular breeding for sesame, but will also serve as a reference for positioning sequence scaffolds on a physical map, to assist in the process of assembling the sesame genome sequence.
Highlights
The genetics and molecular biology of sesame has only recently begun to be studied even though sesame is an important oil seed crop
F2 population and the SLAF-seq approach, which allowed the efficient development of a large number of polymorphic markers in a short time
Genotyping by sequencing is a highthroughput technique that opens the door for the efficient development of large numbers of markers in a short time to generate enough polymorphic markers for high-density genetic map construction
Summary
The genetics and molecular biology of sesame has only recently begun to be studied even though sesame is an important oil seed crop. A high-density genetic map for sesame has not been published yet due to a lack of sufficient molecular markers. SLAF-seq was employed in this study to obtain sufficient markers to construct a high-density genetic map for sesame. Largescale microsatellite marker development is an important approach to obtain sufficient numbers of markers to construct a high-density genetic map. This approach was used for tobacco (Nicotiana tabacum L.) [8] and Japanese flounder (Paralichthys olivaceus) [5], constructing highdensity genetic map with an average interval of less than
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