Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing

Abstract

The human GLI3 protein has a dual function as a transcriptional activator or repressor of hedgehog signaling, depending on the proteolytic processing forms of GLI3. In this study, we established a compound heterozygous GLI3 mutant human embryonic stem cell line (WAe001-A-20) through CRISPR/Cas9 editing. The WAe001-A-20 cells carried two deletions on two different alleles of exon 2 of GLI3, respectively, which resulted in a frame shift and early termination in the translation of GLI3. Moreover, WAe001-A-20 maintains a normal karyotype, parental cell morphology, pluripotent phenotype and the ability to differentiate into three germ layers.Resource tableUnlabelled TableUnique stem cell line identifierWAe001-A-20Alternative name(s) of stem cell lineH1-GLI3-KOInstitutionGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesContact information of distributorYin-xiong Li, li_yinxiong@gibh.ac.cnType of cell lineEmbryonic stem cell lineOriginWAe001-AAdditional origin infoAge: blastocyst stageSex: male, 46, XYCell SourceN/AClonalityClonalMethod of reprogrammingN/AGenetic ModificationYESType of ModificationDeletionAssociated diseaseGreig Cephalopolysyndactyly Syndrome, Pallister-Hall Syndrome, Postaxial PolydactylyGene/locusGLI3/7p14.1Method of modificationCRISPR/Cas9Name of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateApril 20, 2018Cell line repository/bankN/AEthical approvalCell line was used according to institutional ethical guidelines of GIBH