Abstract
Pyropia yezoensis is an economically important seaweed but its molecular genetics is poorly understood. In the present study, we used a doubled haploid (DH) population that was established in our previous work to construct a genetic linkage map of P. yezoensis and analyze the quantitative trait loci (QTLs) of blades. The DH population was genotyped with fluorescent sequence-related amplified polymorphism (SRAP) markers. A chi-square test identified 301 loci with normal segregation (P ≥ 0.01) and 96 loci (24.18%) with low-level skewed segregation (0.001 ≤ P < 0.01). The genetic map was constructed after a total of 92 loci were assembled into three linkage groups (LGs). The map spanned 557.36 cM covering 93.71% of the estimated genome, with a mean interlocus space of 6.23 cM. Kolmogorov-Smirnov test (α = 5%) showed a uniform distribution of the markers along each LG. On the genetic map, 10 QTLs associated with five economic traits of blades were detected. One QTL was for length, one for width, two for fresh weight, two for specific growth rate of length and four for specific growth rate of fresh weight. These QTLs could explain 2.29–7.87% of the trait variations, indicating that their effects were all minor. The results may serve as a framework for future marker-assisted breeding in P. yezoensis.
Highlights
Pyropia yezoensis is a marine red alga with high nutritional values and is one of the most important maricultural crops across the world, mainly in Japan, Korea and China [1]
Genotyping by means of capillary electrophoresis directly provided the digitized information of the fragments (Table 2), which was more efficient than PAGE method [64]
Data of 79 primer combinations with missing data number less than or equal to four doubled haploid (DH) strains per primer combination were used for genetic mapping
Summary
Pyropia yezoensis is a marine red alga with high nutritional values and is one of the most important maricultural crops across the world, mainly in Japan, Korea and China [1]. During the cultivation of P. yezoensis, hundreds of tons of nutrients (nitrogen and phosphorus) are removed from the eutrophic seawater by blade harvest every year [2]. Some problems such as germplasm degeneration, frequent diseases and bad harvests [3,4,5] have arisen under the influence of global warming [6]. Genetic mapping and QTL analysis in Pyropia yezoensis
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