Abstract
Pacific abalone (Haliotis discus hannai) is a commercially important high valued molluscan species. Its wild population has decreased in recent years. It is widely cultured in Korea. Traditional breeding programs have been implemented for hatchery production of abalone seeds. To obtain more genetic information for its molecular breeding program, a high-density linkage map and quantitative trait locus (QTL) for three growth-related traits was constructed for Pacific abalone. F1 cross population with two parents were sampled to construct the linkage map using genotyping by sequencing (GBS). A total of 664,630,534 clean reads and 56,686 SNPs were generated and 3,345 segregating SNPs were used to construct a consensus linkage map. The map spanned 1,747.023 cM with 18 linkage groups and an average interval of 0.55 cM. QTL analysis revealed two significant QTL in LG10 on the consensus linkage map of each growth-related trait. Both QTLs were located in the telomere region of the chromosome. Moreover, four potential candidate genes for growth-related traits were identified in the QTL region. Expression analysis revealed that these identified genes are involved in growth regulation of abalone. The newly constructed genetic linkage map, growth-related QTLs and potential candidate genes identified in the present study can be used as valuable genetic resources for marker-assisted selection (MAS) of Pacific abalone in molecular breeding program.
Highlights
Abalones are marine molluscan species distributed worldwide along coastal waters in tropical and temperate regions (Gordon and Cook, 2013)
The present study reported a single nucleotide polymorphism (SNP) marker based genetic linkage map that was constructed for Pacific abalone using genotyping by sequencing (GBS) approach
GBS is an efficient and cost-effective method that could be used to develop SNP markers, construct genetic linkage map and select marker-based complex traits of many species with or without a reference genome (Elshire et al, 2011; Nie et al, 2017; Liu et al, 2020). This is the first report of quantitative trait locus (QTL) mapping using GBS to screen high quality and abundant SNP markers for constructing a genetic linkage map for Pacific abalone
Summary
Abalones are marine molluscan species distributed worldwide along coastal waters in tropical and temperate regions (Gordon and Cook, 2013). Because of its extraordinary food value due to the presence of bioactive molecules and Growth-Related QTL in Pacific Abalone high price, commercial aquaculture of abalone has expanded widely (Park and Kim, 2013; Hossen et al, 2021). It is reared in sea cages and extensive case farming has distributed along small bays and islands of South, South-West and West coasts of Korea (Choi et al, 2015). As of 2015, abalone production reached about 10,090 metric tons. It is essential to ascertain molecular breeding for Pacific abalone
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