Abstract
BackgroundYellow mustard (Sinapis alba L.) is an important condiment crop for the spice trade in the world. It has lagged behind oilseed Brassica species in molecular marker development and application. Intron length polymorphism (ILP) markers are highly polymorphic, co-dominant and cost-effective. The cross-species applicability of ILP markers from Brassica species and Arabidopsis makes them possible to be used for genetic linkage mapping and further QTL analysis of agronomic traits in yellow mustard.ResultsA total of 250 ILP and 14 SSR markers were mapped on 12 linkage groups and designated as Sal01-12 in yellow mustard. The constructed map covered a total genetic length of 890.4 cM with an average marker interval of 3.3 cM. The QTL for erucic content co-localized with the fatty acid elongase 1 (FAE1) gene on Sal03. The self-(in)compatibility gene was assigned to Sal08. The 4-hydroxybenzyl, 3-indolylmethyl and 4-hydroxy-3-indolylmethyl glucosinolate contents were each controlled by one major QTL, all of which were located on Sal02. Two QTLs, accounting for the respective 20.4% and 19.2% of the total variation of 2-hydroxy-3-butenyl glucosinolate content, were identified and mapped to Sal02 and Sal11. Comparative synteny analysis revealed that yellow mustard was phylogenetically related to Arabidopsis thaliana and had undergone extensive chromosomal rearrangements during speciation.ConclusionThe linkage map based on ILP and SSR markers was constructed and used for QTL analysis of seed quality traits in yellow mustard. The markers tightly linked with the genes for different glucosinolate components will be used for marker-assisted selection and map-based cloning. The ILP markers and linkage map provide useful molecular tools for yellow mustard breeding.
Highlights
Yellow mustard (Sinapis alba L.) is an important condiment crop for the spice trade in the world
The objectives of the present study were 1) to construct a genetic linkage map based on Intron length polymorphism (ILP) and simple sequence repeat (SSR) markers using the F2 population derived from homozygote parental lines, 2) to identify quantitative trait loci (QTL) for erucic acid content and GSL components, and 3) to assign the fatty acid elongase 1 (FAE1) and self-(in) compatibility genes to the respective linkage groups in yellow mustard
Polymorphism between the parental lines Y514 and Y517 A total of 1726 ILP primers – 383 from A. thaliana [10], 1093 from B. napus and 250 from B. rapa available in the Potential Intron Polymorphism (PIP) database [18]
Summary
Yellow mustard (Sinapis alba L.) is an important condiment crop for the spice trade in the world. It has lagged behind oilseed Brassica species in molecular marker development and application. The cross-species applicability of ILP markers from Brassica species and Arabidopsis makes them possible to be used for genetic linkage mapping and further QTL analysis of agronomic traits in yellow mustard. Condiment yellow mustard varieties contain a desirable high 4-hydroxybenzyl (HBEN) glucosinolate (GSL) component in the seed [3], Genetic linkage mapping has proven to be very useful for analyzing quantitative trait loci (QTL), tagging and cloning genes controlling desirable agronomic traits and studying genome organization and evolution. QTL analysis identified two major QTLs for erucic acid content in
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