Abstract
Plasmid pXL1635 was constructed from the already segregationally stable incP-derived pRK290. Plasmid pXL1635 should be suitable for industrial and environmental uses in Gram − bacteria since ( i) it contains the par fragment from RP4 which increases its stability in Pseudomonas denitrificans, a cobalamin-producing and industrially used bacterium, and ( ii) the RK2 oriT has been deleted, leading to a non-mobilizable plasmid.
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