Abstract
A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. The pHT-FabL40 plasmid is stable in both gram-positive and gram-negative hosts with increased plasmid DNA yield in E. coli.
Highlights
A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector
A comparison of triclosan-mediated growth showed that growth of E. coli DH5α and B. subtilis subsp. 168 was inhibited at 0.125 μg/ml and 2 μg/ml triclosan, respectively, versus 3.125 μg/ml ampicillin for E. coli DH5α and 6.25 μg/ml chloramphenicol for B. subtilis subsp. 168 (Figure 1)
These results indicated a higher susceptibility of E. coli DH5α and B. subtilis subsp. 168 to triclosan compared to ampicillin and chloramphenicol, respectively
Summary
A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. 168 was inhibited at 0.125 μg/ml and 2 μg/ml triclosan, respectively, versus 3.125 μg/ml ampicillin for E. coli DH5α and 6.25 μg/ml chloramphenicol for B. subtilis subsp. Ali and Chew (2015) suggested using the FabV protein, a functional homolog of FabI in Vibrio cholera, which confers resistance towards triclosan, for the selection of medium-copy-number plasmids in E. coli.
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