Abstract
BackgroundLittle data is available on prognostic biomarkers and effective treatment options for Kidney Renal Papillary Cell Carcinoma (KIRP) patients, to find potential prognostic biomarkers and new targets was an urgent mission for KIRP therapy.MethodsThe differentially expressed autophagy-related genes (DEARGs) were screened out according to the RNA sequencing data in The Cancer Genome Atlas database, then identified survival-related DEARGs to establish a prognostic model for survival predicting of KIRP patients. Then we verified the robustness and validity of the prognostic risk model through clinicopathological data. At last, we evaluate the prognostic value of genes that formed the prognostic risk model individually.ResultsWe analyzed the expression of 232 autophagy-related genes (ARGs) in 289 KIRP and 32 non-tumor tissue cases, and 40 mRNAs were screened out as DEARGs. The functional and pathway enrichment analysis was done and protein–protein interaction network was constructed for all DEARGs. To sift candidate DEARGs associated with KIRP patients’ survival and create an autophagy-related risk prognostic model, univariate and multivariate Cox regression analysis were did separately. Eventually 3 desirable independent prognostic DEARGs (P4HB, NRG1, and BIRC5) were picked out and used for construct the autophagy-related risk model. The accuracy of the prognostic risk model for survival prediction was assessed by Kaplan–Meier plotter, receiver-operator characteristic curve, and clinicopathological correlational analyses. The prognostic value of above 3 genes was verified individually by survival analysis and expression analysis on mRNA and protein level.ConclusionsThe autophagy-related prognostic model is accurate and applicable, it can predict OS independently for KIRP patients. Three independent prognostic DEARGs can benefit for facilitate personalized target treatment too.
Highlights
Little data is available on prognostic biomarkers and effective treatment options for Kidney Renal Papillary Cell Carcinoma (KIRP) patients, to find potential prognostic biomarkers and new targets was an urgent mission for KIRP therapy
Three independent prognostic differentially expressed autophagy-related genes (DEARGs) can benefit for facilitate personalized target treatment too
Individual assessment of the function of 3 prognostic-related DEARGs in KIRP further proof P4HB, BIRC5, and NRG1 are up-regulated in KIRP, and overexpression of them is associated with worse survival of KIRP patients
Summary
Little data is available on prognostic biomarkers and effective treatment options for Kidney Renal Papillary Cell Carcinoma (KIRP) patients, to find potential prognostic biomarkers and new targets was an urgent mission for KIRP therapy. Kidney Renal Papillary Cell Carcinoma (KIRP) is the second most commonly diagnosed subtype of RCC which accounting for 15– 20% of RCC cases, and the most common subtype is clear cell renal carcinoma (ccRCC) [3, 4]. As most KIRP patients had a good prognosis before metastasis, and m-KIRP patients are not so many in contrast to ccRCC, there is little data on the efficacy of available treatment options and few prognostic molecular markers have been discovered. There is a need to explore potential prognostic markers and new molecular targets for KIRP therapy
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