Abstract
AbstractThe plant expression vectors pCAMBIA1301PMIand pBIPMIwere constructed by substituting theEscherichia coliphosphomannose-isomerase (PMI) gene for thehptgene of pCAMBIA1301 andgusgene of pBI121, respectively. Epicotyl explants of the Xuegan sweet orange (Citrus sinensisL. Osbeck) were inoculated withAgrobacterium tumefaciensEHA105- pCAMBIA1301PMIand EHA105-pBIPMIand subsequently selected in a medium supplemented with a combination of 25 g/l mannose and 5 g/l sucrose as the carbon source. The transformation efficiency rate was 27.7% when transformed by pCAMBIA1301PMIand 12.7% by pBIPMI. Genetic transformation was confirmed by chlorophenol red assay and polymerase chain reaction (PCR). A new method for obtaining transgenic Xuegan sweet orange plants was developed using thePMI/mannose selection system.
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