Abstract
Methods for detection ofCandida albicansin culture or biological samples were developed by the use of polymerase chain reaction (PCR) with oligonucleotide primers fromC. albicans70 kDa heat shock protein gene (Cahsp70). The PCR amplifies a 335-base pair fragment which is then hybridized with a non-radioactive probe, leading to the specific identification ofC. albicansand its differentiation from all other human pathogenicCandidaand/or yeast species.Candida albicanscould be rapidly detected in human urine and blood, with a sensitivity of 10 and 50 fungal cells per sample, respectively.
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