Construction and Titration of a Recombinant Canine Adenovirus Expressing the Rabies G Protein

Abstract

To prevent rabies in domestic animals we need safe and effective vaccines. Although there are commercial inactivated vaccines in place to ensure protection against rabies in swine, but searching for a more economically viable formulation for use in developed countries is always a priority. In this paper we designed a structural model of a rabies vaccine using the molecular cloning techniques represented by a recombinant adenovirus (CAV-2) where was replaced E3 region with the an insert represented by G rabies protein. Using plasmids who contains the canine adenovirus type 2 virus (CAV-2) and rabies glycoprotein which was digested by restriction enzyme such as SwaI, MboI, AscI and NotI we obtained the vector pRecCAV2CMV-G. This vector was transfected in DKcre cells, multiplied and purified. The results after titration in DKcre cells revealed us a 10 8 TCID 50% /ml virus concentration and 10 10,4 infectious particles using real time PCR.