Abstract
Construction and in vitro evaluation of a recombinant live attenuated PRRSV expressing GM-CSF
Highlights
Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be an important problem for the swine industry
Construction and rescue of the recombinant PRRSV Pei et al [26] and Lawson SR et al [15] showed that the position between ORF1b and ORF2 of PRRSV is a suitable site for foreign gene insertion [15,26]
The Granulocyte–macrophage colony stimulating factor (GM-CSF) gene and the synthesized transcription-regulating sequence 6 (TRS6) sequence were inserted between the stop codon of ORF1b and the start codon of ORF2 of the infectious molecular clone of HuN4-F112 vaccine strain by overlap PCR (Figure 1)
Summary
Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be an important problem for the swine industry. Inactivated vaccines and modified-live virus vaccines are widely used in the field; the efficacy of these PRRSV vaccines is suboptimal due to poor immunogenicity. In 2006, a highly virulent strain of PRRSV (HP-PRRSV), which caused significant morbidity can be characterized by high fever, was first detected in China and rapidly spread to most provinces of China [4,5,6]. Under these circumstances, vaccination against PRRSV provides invaluable support to increase host resistance, reduce environmental contamination, and reduce the chance of regional outbreaks. To respond to the recently emerged HP-PRRSV, new live attenuated vaccines were developed by passaging HP-PRRSV isolates in MARC-145 cell lines [7,8,9]
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